Method development of an extraction of polar pesticides from fatty matrix (olives) - Additional clean-up with C18 phase before CHROMABOND® HR-P
Summary
Type: SPE Application #: 304490
Catalog#: 730108 - SPE Cartridges, Chromabond, HR-P, 3ml, PP, 200mg 30/pk
Detection: The extracted sample is determinated by LC/ESI-MS/MS by a polar RP column (e.g. NUCLEODUR® C18 Pyramid) with methanol / 10 mM ammonium acetate gradient. Conditions: Clean-up with CHROMABOND® C18 (recommanded as C18 silica):
Column conditioning: 5 ml water and 5 ml acetonitrile
Sample aspiration: 3 ml extract is passed through C18 column in a tapered flask.
Authors: Pantiru M E Source: Dissertation Wissenschaftszentrum Weihenstephan f&
Details
Legend: Recovery rates of polar pesticides from fatty olive matrix with different acetonitile volumes, determinated by LC-MS/MS (positive ion mode).
The great elution power of acetonitrile cause lower reco